Chromatin three-dimensional structure of rapeseed hybrids

2022-05-12 0 By

A king of coordinating editor | # # Heterosis (Heterosis) is the phenomena of genetic biology, refers to the chimeras in one or more characters (such as the life force, growth potential, fecundity, resistance, yield, quality, etc.) is better than that of the phenomenon of parents.The successful application of heterosis breeding in agricultural production has made great contributions to improving crop yield, solving food crisis and guaranteeing food security.In order to further improve the utilization potential of heterosis and give full play to its important role in leading hybrid breeding and guiding human production, it is of great scientific significance to further study its mechanism, especially the molecular genetic basis.However, most of the existing studies focused on the effect of genetic variation caused by DNA sequence at the one-dimensional level on progeny heterosis, but ignored the effect of chromatin on heterosis in the three-dimensional space. The mechanism behind heterosis is still not fully understood.Photo source:Baidu recently,Comparison of Dynamic 3D Chromatin Architecture Uncovers was published by Prof. Jinxing Tu and Prof. Qingyong Yang in the international academic Journal Of Advanced ResearchIn this paper, heterosis for Leaf size in Brassica napus is the first to explore the underlying mechanism of heterosis in Brassica napus from the perspective of chromatin three-dimensional structure.In this study, two combinations of strong and weak heterosis were selected by constructing a b. napus population.Using 3d genome, whole genome resequencing, transcriptomic and phenotypic data, the potential effects of 3d genome structure on seedling heterosis in rapeseed were investigated.Compartment, 19 — 21% of the three dimensional chromatin structure of the F1 hybrid is changed compared with that of the parent.And the strong dominant hybrids had more a-loci (transcriptionally active, more open chromatin) than the weak dominant hybrids.Further studies showed that the genetic differences between the three dimensional chromatin structure of the hybrid and the parents were significantly co-located.In addition, the relatively open three-dimensional chromatin structure in the strongly dominant hybrids resulted in a higher proportion (46 — 64%) of genes with expression dominance than those in the parents, compared with the weak dominant hybrids (22 — 31%).Interestingly, it was also found that the relatively open chromatin three-dimensional structure in the highly dominant hybrids resulted in more up-regulated auxin related genes.By measuring plant hormones and cytological experiments, it was found that the increase of auxin content accelerated the cell cycle of strong and dominant hybrids, and then promoted the proliferation and enlargement of leaf cells, resulting in larger leaf area.In conclusion, this study analyzed the potential mechanism of genetic differences between parents on heterosis formation from the perspective of chromatin three-dimensional structure, and provided a new idea for exploring the biological basis of heterosis formation in plants.Hu Yue, Ph.D., College of Information Science and Technology, Huazhong Agricultural University, and Xiong Jie, PhD., College of Plant Science and Technology, Huazhong Agricultural University, were co-first authors of this paper.Qingyong Yang, Professor, College of Information Science, Huazhong Agricultural University, and Jinxing Tu, Professor, College of Plant Science and Technology are the co-corresponding authors of this paper.The research was supported by the National Key RESEARCH and Development Program, hubei Provincial Science and Technology Major Special Project, National Natural Science Foundation of China, Hubei Provincial Natural Science Foundation and other projects.Paper links: https://authors.elsevier.com/sd/article/S2090-1232 (22), 00001-7